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p gp function  (MedChemExpress)


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    MedChemExpress p gp function
    P Gp Function, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p gp function/product/MedChemExpress
    Average 94 stars, based on 3 article reviews
    p gp function - by Bioz Stars, 2026-02
    94/100 stars

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    Endothelial cells express functional P-gp protein. Panel a and b : Verapamil blocks Rho efflux in endothelial cells. Dox- or noninduced HMEC and HUVEC cells were incubated in the absence or presence of Rho for 1 hour at 37°C. Rho accumulation was inhibited by the addition of Verapamil (Vrp) at 30 μM to the cell incubation mixture. The cells were analyzed in the flow cytometer. Panel c and d : Activity of endothelial P-gp is blocked by Verapamil and MRK16. Parental HMEC, HMECd1, and HMECd2 cells were incubated with 1 μM (c) or 2 μM (d) Rho for 1 hour at 37°C in the absence or presence of 30 μM Verapamil or 10 μg/ml MRK16 (MRK). Rho accumulation was measured by flow cytometry and quantified as the MFI. The background fluorescence level, determined using cells not exposed to Rho, was subtracted from the data. Results are expressed as the mean±SEM of 3 separate experiments. *p < 0.025, **p < 0.01.

    Journal: Journal of Hematology & Oncology

    Article Title: Induction of acquired drug resistance in endothelial cells and its involvement in anticancer therapy

    doi: 10.1186/1756-8722-6-49

    Figure Lengend Snippet: Endothelial cells express functional P-gp protein. Panel a and b : Verapamil blocks Rho efflux in endothelial cells. Dox- or noninduced HMEC and HUVEC cells were incubated in the absence or presence of Rho for 1 hour at 37°C. Rho accumulation was inhibited by the addition of Verapamil (Vrp) at 30 μM to the cell incubation mixture. The cells were analyzed in the flow cytometer. Panel c and d : Activity of endothelial P-gp is blocked by Verapamil and MRK16. Parental HMEC, HMECd1, and HMECd2 cells were incubated with 1 μM (c) or 2 μM (d) Rho for 1 hour at 37°C in the absence or presence of 30 μM Verapamil or 10 μg/ml MRK16 (MRK). Rho accumulation was measured by flow cytometry and quantified as the MFI. The background fluorescence level, determined using cells not exposed to Rho, was subtracted from the data. Results are expressed as the mean±SEM of 3 separate experiments. *p < 0.025, **p < 0.01.

    Article Snippet: Antibody MRK16 blocking P-gp function was obtained from Kamiya Biomedical Company (Seattle, WA).

    Techniques: Functional Assay, Incubation, Flow Cytometry, Activity Assay, Fluorescence